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Site-Specific Dual Functionalization of Cysteine Residue in Peptides and Proteins with 2-Azidoacrylates.

Shinya AriyasuHirohito HayashiBengang XingShunsuke Chiba
Published in: Bioconjugate chemistry (2017)
Herein, we report use of 2-azidoacrylates to perform site-specific dual functionalization of the cysteine residue of peptides and bovine serum albumin (BSA), a native protein containing one free cysteine residue. The sulfhydryl group of the cysteine residue could be conjugated with 2-azidoacrylates bearing various functionalities, such as fluorescent dyes under physiological aqueous buffer conditions, to afford peptide and protein conjugates anchoring an azide moiety. Successive azide-alkyne cycloaddition enables installation of the second functionality, thus affording dual-functionalized peptide- and protein-based materials.
Keyphrases
  • amino acid
  • living cells
  • fluorescent probe
  • protein protein
  • quantum dots
  • ionic liquid
  • simultaneous determination
  • transition metal