Parallel and comparative non-targeted metabolomic speciation analysis of metalloids and their non-metal analogues by HPLC-ICPMS/MS in mushrooms.
Bassam LajinSimone BraeuerWalter GoesslerPublished in: Metallomics : integrated biometal science (2022)
With the introduction of tandem mass spectrometry to inductively coupled plasma mass spectrometry (ICPMS/MS), the potential for non-targeted elemental metabolomic analysis has been expanded to many non-metals of pivotal biological importance. Arsenic and selenium are trace elements that share chemical similarity with the non-metals phosphorus and sulfur, respectively, and this similarity can be exploited to gain more insight into the incompletely understood biological significance of these metalloids and the evolution of their biochemical pathways. As a proof of concept, we show the applicability of HPLC-ICPMS/MS for non-targeted and parallel speciation analysis of arsenic, selenium, phosphorus, and sulfur in mushrooms-metabolically diverse organisms. Incredibly contrasting levels of diversity were found in the metabolomic profiles of the four investigated elements among the various species along with sharp discrepancies among related elements (e.g. phosphorous vs. arsenic) in certain mushroom species. The present work shows that ICPMS/MS offers a new dimension in non-targeted metabolomic analysis and enables a unique comparative approach in investigating and tracking the biochemistry of related elements in moderately complex organisms.
Keyphrases
- mass spectrometry
- high performance liquid chromatography
- liquid chromatography
- tandem mass spectrometry
- ms ms
- gas chromatography
- simultaneous determination
- ultra high performance liquid chromatography
- capillary electrophoresis
- cancer therapy
- high resolution mass spectrometry
- multiple sclerosis
- high resolution
- drinking water
- solid phase extraction
- heavy metals
- human health
- health risk
- risk assessment
- health risk assessment
- multidrug resistant
- data analysis