Insufficient repeatability and reproducibility of MALDI-TOF MS-based identification of MRSA.
Veronika PaskovaKaterina ChudejovaAnna SramkovaLucie KraftovaVladislav JakubuEfthimia A PetinakiHelena ZemlickovaKaterina NeradovaCostas C PapagiannitsisJaroslav HrabakPublished in: Folia microbiologica (2020)
Rapid identification of methicillin-resistant Staphylococcus aureus (MRSA) is essential for proper initial antibiotic therapy and timely set up of hygienic measures. Recently, detection of MRSA using MALDI-TOF mass spectrometer mediated by the peptide-phenol-soluble modulin (PSM-mec)-linked to the class A mec gene complex present in SCCmec cassettes types II, III, and VIII of MRSA strains, has been commercially available. We present here a multicentre study on MALDI-TOF MS detection of MRSA evincing a poor repeatability and reproducibility of the assay. The sensitivity of the assay varies between 50 and 90% in strains carrying psmMEC and psmδ genes encoding for PSM-mec and δ-toxin (a member of the PSM peptide family), respectively. No false positive results were found. The very major error calculation was 30% and the major error achieved 0%. Interlaboratory repeatability varies between 0 and 100%. No significant difference was observed with the use of different cultivation media. Our data showed a poor sensitivity of the method excluding it from the use in routine laboratory testing.
Keyphrases
- methicillin resistant staphylococcus aureus
- mass spectrometry
- staphylococcus aureus
- escherichia coli
- loop mediated isothermal amplification
- genome wide
- bioinformatics analysis
- high throughput
- high resolution
- ms ms
- real time pcr
- stem cells
- clinical practice
- dna methylation
- genome wide identification
- machine learning
- big data
- transcription factor
- cell therapy
- smoking cessation