Login / Signup

Catalytic hairpin assembly-based electrochemical biosensor with tandem signal amplification for sensitive microRNA assay.

Lin CuiJinghua ZhouXiao-Yun YangJing DongXiaolei WangShuangshuang Zhang
Published in: Chemical communications (Cambridge, England) (2021)
We demonstrate for the first time the construction of a low background electrochemical biosensor with tandem signal amplification for sensitive microRNA assay based on target-activated catalytic hairpin assembly (CHA) of heteroduplex-templated copper nanoparticles. This electrochemical biosensor exhibits high sensitivity, good specificity, single-base mismatch discrimination capability, excellent stability and reproducibility, and it can sensitively detect microRNA in cancer cells.
Keyphrases
  • label free
  • gold nanoparticles
  • high throughput
  • ionic liquid
  • nucleic acid
  • molecularly imprinted
  • quantum dots
  • high resolution