Kaempferia parviflora Extracts Protect Neural Stem Cells from Amyloid Peptide-Mediated Inflammation in Co-Culture Model with Microglia.
Piya TemviriyanukulAnchana ChansawhangJirarat KarinchaiSataporn PhochantachindaShutipen BuranasinsupWoorawee InthachatPornsiri PitchakarnBoonrat ChantongPublished in: Nutrients (2023)
The existence of neuroinflammation and oxidative stress surrounding amyloid beta (Aβ) plaques, a hallmark of Alzheimer's disease (AD), has been demonstrated and may result in the activation of neuronal death and inhibition of neurogenesis. Therefore, dysregulation of neuroinflammation and oxidative stress is one possible therapeutic target for AD. Kaempferia parviflora Wall. ex Baker (KP), a member of the Zingiberaceae family, possesses health-promoting benefits including anti-oxidative stress and anti-inflammation in vitro and in vivo with a high level of safety; however, the role of KP in suppressing Aβ-mediated neuroinflammation and neuronal differentiation has not yet been investigated. The neuroprotective effects of KP extract against Aβ 42 have been examined in both monoculture and co-culture systems of mouse neuroectodermal (NE-4C) stem cells and BV-2 microglia cells. Our results showed that fractions of KP extract containing 5,7-dimethoxyflavone, 5,7,4'-trimethoxyflavone, and 3,5,7,3',4'-pentamethoxyflavone protected neural stem cells (both undifferentiated and differentiated) and microglia activation from Aβ 42 -induced neuroinflammation and oxidative stress in both monoculture and co-culture system of microglia and neuronal stem cells. Interestingly, KP extracts also prevented Aβ 42 -suppressed neurogenesis, possibly due to the contained methoxyflavone derivatives. Our data indicated the promising role of KP in treating AD through the suppression of neuroinflammation and oxidative stress induced by Aβ peptides.
Keyphrases
- oxidative stress
- cerebral ischemia
- diabetic rats
- induced apoptosis
- stem cells
- lipopolysaccharide induced
- inflammatory response
- lps induced
- subarachnoid hemorrhage
- neural stem cells
- dna damage
- ischemia reperfusion injury
- traumatic brain injury
- brain injury
- blood brain barrier
- cognitive impairment
- public health
- healthcare
- signaling pathway
- mental health
- climate change
- cognitive decline
- social media
- spinal cord injury
- mild cognitive impairment
- endoplasmic reticulum stress
- bone marrow
- cell death
- spinal cord
- cell proliferation
- heat shock
- endothelial cells