Effects of Silver Diamine Nitrate and Silver Diamine Fluoride on Dentin Remineralization and Cytotoxicity to Dental Pulp Cells: An In Vitro Study.
Surapong SrisomboonMatana KettratadAndrew StrayPhakkhananan PakawanitCatleya RojviriyaSomying PatntirapongPiyaphong PanpisutPublished in: Journal of functional biomaterials (2022)
Silver diamine nitrate (SDN) is expected to help control caries similar to silver diamine fluoride (SDF). The aim of this study was to determine the mineral precipitation in demineralized dentin and the cytotoxicity of SDN and SDF to dental pulp cells. Demineralized dentin specimens were prepared, and SDF, SDN, or water (control) was applied. The specimens were then remineralized in simulated body fluid for 2 weeks. The mineral precipitation in the specimens was examined using FTIR-ATR, SEM-EDX, and synchrotron radiation X-ray tomographic microscopy (SRXTM). Additionally, the cytotoxicity of SDF and SDN to human dental pulp stem cells was analyzed using an MTT assay. The increase in FTIR spectra attributable to apatite formation in demineralized dentin in the SDF group was significantly higher compared to the SDN and control groups ( p < 0.05). Dentinal tubule occlusion by the precipitation of silver salts was detected in both SDF and SDN groups. The mineral density as shown in SRXTM images and cytotoxicity of both SDN and SDF groups were comparable ( p > 0.05). In conclusion, SDF demonstrated superior in vitro apatite formation compared to SDN. However, the degree of mineral precipitation and cytotoxic effects of both were similar.
Keyphrases
- gold nanoparticles
- stem cells
- induced apoptosis
- silver nanoparticles
- drinking water
- cell cycle arrest
- endothelial cells
- nitric oxide
- high resolution
- high throughput
- optical coherence tomography
- oxidative stress
- radiation therapy
- deep learning
- magnetic resonance imaging
- cell death
- mass spectrometry
- radiation induced
- computed tomography
- density functional theory
- preterm birth
- oral health
- dna repair