Development and evaluation of time-resolved fluorescent immunochromatographic assay for quantitative detection of SARS-CoV-2 spike antigen.

Buzhou XuHao TangYiming WengValerie Sloane JonesShuhong LuoChih Yun ChoYongping LinJianmin FangXuedong SongRuo-Pan Huang

Published in: Journal of clinical laboratory analysis (2022)

The new S1-RBD antigen test is a rapid (15 min), sensitive, and specific assay that requires minimal sample preparation. Critically, the assay correlated closely with PCR-based methodology in nasopharyngeal swab samples, showing that the detected S1-RBD antigen levels correlate with SARS-CoV-2 virus load. Therefore, the new TRF lateral flow test for S1-RBD has potential application in point-of-care settings.

Keyphrases

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respiratory syndrome coronavirus
loop mediated isothermal amplification
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quantum dots
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climate change