Matrix-free human pluripotent stem cell manufacturing by seed train approach and intermediate cryopreservation.

Kevin UllmannFelix MansteinWiebke TriebertNils KriedemannAnnika FrankeJana TeskeMira MertensVictoria LupanowGudrun GöhringAlexandra HaaseUlrich MartinRobert Zweigerdt

Published in: Stem cell research & therapy (2024)

Together, an entirely matrix-free, highly efficient, flexible and automation-friendly hPSC expansion strategy is demonstrated, facilitating the development of good manufacturing practice-compliant closed-system manufacturing in large scale.

Keyphrases

highly efficient
stem cells
endothelial cells
primary care
healthcare
induced pluripotent stem cells
high speed
quality improvement
mass spectrometry
cell therapy
bone marrow
low cost
high resolution