Label-Free SERS and MD Analysis of Biomarkers for Rapid Point-of-Care Sensors Detecting Head and Neck Cancer and Infections.
Edoardo FarnesiSilvia RinaldiChen LiuJonas PrengelOrlando Guntinas-LichiusMichael SchmittDana Cialla-MayJuergen PoppPublished in: Sensors (Basel, Switzerland) (2023)
For the progress of point-of-care medicine, where individual health status can be easily and quickly monitored using a handheld sensor, saliva serves as one of the best-suited body fluids thanks to its availability and abundance of physiological indicators. Salivary biomarkers, combined with rapid and highly sensitive detection tools, may pave the way to new real-time health monitoring and personalized preventative therapy branches using saliva as a target matrix. Saliva is increasing in importance in liquid biopsy, a non-invasive approach that helps physicians diagnose and characterize specific diseases in patients. Here, we propose a proof-of-concept study combining the unique specificity in biomolecular recognition provided by surface-enhanced Raman spectroscopy (SERS) in combination with molecular dynamics (MD) simulations, which give leave to explore the biomolecular absorption mechanism on nanoparticle surfaces, in order to verify the traceability of two validated salivary indicators, i.e., interleukin-8 (IL-8) and lysozyme (LYZ), implicated in oropharyngeal squamous cell carcinoma (OSCC) and oral infection. This strategy simultaneously assures the detection and interpretation of protein biomarkers in saliva, ultimately opening a new route for the evolution of fast and accurate point-of-care SERS-based sensors of interest in precision medicine diagnostics.
Keyphrases
- molecular dynamics
- sensitive detection
- loop mediated isothermal amplification
- label free
- raman spectroscopy
- quantum dots
- squamous cell carcinoma
- density functional theory
- end stage renal disease
- chronic kidney disease
- gold nanoparticles
- primary care
- healthcare
- ejection fraction
- newly diagnosed
- peritoneal dialysis
- low cost
- prognostic factors
- high resolution
- radiation therapy
- binding protein
- health information
- climate change
- mesenchymal stem cells
- cystic fibrosis
- lymph node metastasis
- locally advanced
- small molecule
- ionic liquid
- ultrasound guided
- human health
- rectal cancer
- patient reported
- candida albicans