Use of real-time multiplex PCR, malaria rapid diagnostic test and microscopy to investigate the prevalence of Plasmodium species among febrile hospital patients in Sierra Leone.
Tomasz A LeskiChris Rowe TaittAbdulai G SwarayUmaru BanguraNathanael D ReynoldsAndrew HoltzChadwick YasudaJoseph LahaiJoseph M LaminVictoria BaioKathryn H JacobsenRashid AnsumanaDavid A StengerPublished in: Malaria journal (2020)
Wider application of PCR for detection of malaria parasites may help to fill gaps existing as a result of use of microscopy and RDTs. Due to its high sensitivity and specificity, species coverage, room temperature stability and relative low complexity, the MMSR assay may be useful for detection of malaria and epidemiological studies especially in low-resource settings.
Keyphrases
- plasmodium falciparum
- real time pcr
- room temperature
- high throughput
- label free
- loop mediated isothermal amplification
- end stage renal disease
- high resolution
- single molecule
- ejection fraction
- newly diagnosed
- high speed
- chronic kidney disease
- healthcare
- optical coherence tomography
- ionic liquid
- risk factors
- prognostic factors
- peritoneal dialysis
- emergency department
- single cell
- mass spectrometry
- patient reported outcomes
- acute care