Multiple approaches for massively parallel sequencing of SARS-CoV-2 genomes directly from clinical samples.
Minfeng XiaoXiaoqing LiuJingkai JiMin LiJiandong LiLin YangWanying SunPeidi RenGuifang YangJincun ZhaoTianzhu LiangHuahui RenTian ChenHuanzi ZhongWenchen SongYanqun WangZiqing DengYanping ZhaoZhihua OuDaxi WangJielun CaiXinyi ChengTaiqing FengHonglong WuYanping GongHuanming YangJian WangXun XuShida ZhuFang ChenYanyan ZhangWeijun ChenYimin LiJunhua LiPublished in: Genome medicine (2020)
This is, to the best of our knowledge, the first work systematically investigating inter- and intra-individual variations of SARS-CoV-2 using amplicon- and capture-based whole-genome sequencing, as well as the first comparative study among multiple approaches. Our work offers practical solutions for genome sequencing and analyses of SARS-CoV-2 and other emerging viruses.