Chromatographic analysis of ciprofloxacin and metronidazole in real human plasma: green analytical chemistry perspective.

Background: Ciprofloxacin and metronidazole are beneficial for treating mixed aerobic/anaerobic infections. Methods: Following the oral administration of ciprofloxacin and metronidazole in healthy volunteers, TLC and HPLC methods were described for their analysis in plasma samples. In the first method, a stationary phase of silica gel TLC F254 plates was used using acetone/water/triethylamine/glacial acetic acid (8:2:0.25:0.1 v/v). The second approach used a C18 column and methanol/aqueous 0.05% triethylamine (25:75 v/v), with a flow rate of 1 ml/min and detection at 325 nm. Four green metrics were used to evaluate the approaches' environmental impact. Conclusion: The study provided the sensitivity required for determination of the two drugs in the collected samples. The findings showed that results were within permitted ranges with minimal environmental impact.