Nitric oxide inhibition of lipopolysaccharide-stimulated RAW 247.6 cells by ibuprofen-conjugated iron oxide nanoparticles.
Mariana RomanoMayara Klimuk UchiyamaRoberta M CardosoSergio Hiroshi TomaMauricio da Silva BaptistaKoiti ArakiPublished in: Nanomedicine (London, England) (2020)
Aim: To develop a series of superparamagnetic iron oxide nanoparticles (SPIONs) by coconjugating them with ibuprofen (ibu) and glycerol phosphate (glycerol) or ibu and glucose-1-phosphate and to assess capacity of these conjugates to inhibit the release of nitric oxide (NO) in macrophages, even at low concentrations. Materials & methods: The SPION conjugates were characterized and their properties evaluated showing the influence of those ligands on colloidal stability and inhibition of NO-release demonstrated. The cytotoxicity and possible anti-inflammatory activity were evaluated using murine macrophages (RAW 247.6). Results: SPION-glycerol phosphate/ibu conjugates inhibited the NO production induced by lipopolysaccharides, indicating a potential anti-inflammatory activity. Conclusion: SPION conjugated with ibu was shown to inhibit NO-release even at very low concentrations, suggesting possible action against inflammatory diseases.
Keyphrases
- iron oxide nanoparticles
- nitric oxide
- cancer therapy
- induced apoptosis
- photodynamic therapy
- nitric oxide synthase
- hydrogen peroxide
- cell cycle arrest
- oxidative stress
- toll like receptor
- inflammatory response
- blood pressure
- type diabetes
- blood glucose
- risk assessment
- drug delivery
- postoperative pain
- metabolic syndrome
- cell proliferation
- weight loss
- skeletal muscle
- lps induced
- glycemic control