Clinicopathological characteristics associated with the engraftment of patient lymphoma cells in NOG mice.
Satomi SahashiKazuyuki ShimadaYusuke TakagiTomohiro AokiShunsuke KunouAkihiko SakamotoAtsushi MuraseKatsuya FurukawaYusuke KagayaYusuke YamagaMika TakaiKiyonobu TokuyamaSatoko ShimadaShigeo NakamuraHitoshi KiyoiPublished in: International journal of hematology (2023)
Patient-derived xenograft (PDX) mouse models are useful for deepening our understanding of the biology of malignant lymphoma; however, factors associated with the success of the PDX lymphoma model are largely unknown. We retrospectively analyzed the characteristics of 66 xenotransplantations from 65 patients. In all, 43 (65%) specimens were obtained from patients aged > 60 years, and 42 (64%) specimens were obtained at diagnosis. Specimens were obtained from patients with the following diseases: diffuse large B-cell lymphoma (n = 30), intravascular large B-cell lymphoma (n = 12), follicular lymphoma (n = 8), peripheral T-cell lymphoma (n = 7), mantle cell lymphoma (n = 2), and other (n = 7). The specimens were sourced mainly from bone marrow (n = 31, 47%) and extranodal tumors (n = 13, 20%). Engraftment was successful in 33/66 (50%) xenotransplantations. The median age of patients who provided successful specimens was significantly higher than that for unsuccessful specimens (p = 0.013). Specimens with a high proportion of tumor cells in the graft and those obtained from patients with relapsed/refractory disease showed higher tendencies toward successful engraftment. Taken together, these data suggest that tumor cells with a highly malignant potential might have a high likelihood of engraftment.
Keyphrases
- diffuse large b cell lymphoma
- epstein barr virus
- end stage renal disease
- bone marrow
- fine needle aspiration
- ejection fraction
- newly diagnosed
- chronic kidney disease
- mouse model
- peritoneal dialysis
- prognostic factors
- oxidative stress
- ultrasound guided
- mesenchymal stem cells
- risk assessment
- signaling pathway
- adipose tissue
- electronic health record
- endoplasmic reticulum stress
- insulin resistance
- high fat diet induced
- pi k akt