Creating Optimal Conditions for OPA1 isoforms by Western Blot in Muscle Tissue.
Dominique C StephensAmber CrabtreeHeather K BeasleyEdgar Garza-LopezMargaret MungaiKit NeikirkLarry VangZer VueNeng VueAndrea G MarshallKyrin TurnerJianqiang ShaoSandra MurrayJennifer A GaddyCelestine WanjallaJamaine DavisSteven DamoAntentor O HintonPublished in: bioRxiv : the preprint server for biology (2023)
Samples for western blot analysis are isolated from lysed cells, loaded onto a gel, and ran using optimized conditions to better isolate OPA1 isoforms. Samples are transferred to a membrane to for incubation and protein detection using OPA1 antibodies.