Creating Optimal Conditions for OPA1 isoforms by Western Blot in Muscle Tissue.

Dominique C StephensAmber CrabtreeHeather K BeasleyEdgar Garza-LopezMargaret MungaiKit NeikirkLarry VangZer VueNeng VueAndrea G MarshallKyrin TurnerJianqiang ShaoSandra MurrayJennifer A GaddyCelestine WanjallaJamaine DavisSteven DamoAntentor O Hinton

Published in: bioRxiv : the preprint server for biology (2023)

Samples for western blot analysis are isolated from lysed cells, loaded onto a gel, and ran using optimized conditions to better isolate OPA1 isoforms. Samples are transferred to a membrane to for incubation and protein detection using OPA1 antibodies.

Keyphrases

induced apoptosis
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drug delivery
cell cycle arrest
skeletal muscle
wound healing
protein protein
binding protein
endoplasmic reticulum stress
cancer therapy
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signaling pathway
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hyaluronic acid