Sample and Library Preparation Approaches for the Analysis of the Virome of Irrigation Water.

While virome characterization applied in irrigation waters is an important tool to protect public health by informing on circulating human and zoonotic infections, optimized and standardized procedures should be followed to reduce the variability of results related to either the sample itself and the downstream bioinformatics analyses. Our results show that virome characterization can be an important tool in the discovery of pathogenic viruses in the environment and can be used to inform and optimize reference-based detection methods provided appropriate and rigorous controls are included. This article is protected by copyright. All rights reserved.