OM-LV20, a novel peptide from odorous frog skin, accelerates wound healing in vitro and in vivo.
Xiaojie LiYing WangZhirong ZouMeifeng YangChunyun WuYunshan SuJing TangMeifeng YangPublished in: Chemical biology & drug design (2017)
The healing of chronic wounds remains a considerable challenge in clinical trials and imposes severe financial and physiological burdens on patients. Many works are being tried to find ideal clinical promoting wound healing biomaterials. Small bioactive peptides with low cost and easy production, store and transfer become excellent candidates. Here, we identified a novel peptide (named OM-LV20) from skin secretions of odorous frog Odorrana margaretae. The peptide had an amino acid sequence of "LVGKLLKGAVGDVCGLLPIC," contained an intramolecular disulfide bridge at the C-terminus, and was produced by post-translational processing of a 71-residue prepropeptide. Our results showed that OM-LV20 had no direct microbe-killing effects, hemolytic activity, or acute toxicity, but did exhibit weak antioxidant activity. OM-LV20 promoted wound healing against human keratinocytes (HaCaT) and human skin fibroblasts (HSF) in both time- and dose-dependent manners. In addition, it induced the proliferation of HaCaT but not HSF cells. Of note, OM-LV20 showed strong wound healing-promoting activity in a mice model of full-thickness skin wound. Our research indicates the cellular and animal level wound healing potential of OM-LV20, and thus provides a novel bioactive peptide template for the development of wound healing agents and medicine.
Keyphrases
- wound healing
- amino acid
- clinical trial
- low cost
- drug induced
- oxidative stress
- end stage renal disease
- endothelial cells
- induced apoptosis
- newly diagnosed
- liver failure
- prognostic factors
- adipose tissue
- heat shock
- risk assessment
- open label
- optical coherence tomography
- diabetic rats
- mass spectrometry
- high glucose
- cell death
- young adults
- insulin resistance
- hepatitis b virus
- induced pluripotent stem cells
- aortic dissection
- peritoneal dialysis
- acute respiratory distress syndrome
- phase ii
- high resolution