Parental epigenetic asymmetry of PRC2-mediated histone modifications in the Arabidopsis endosperm.
Jordi Moreno-RomeroHua JiangJuan Santos-GonzálezClaudia KöhlerPublished in: The EMBO journal (2016)
Parental genomes in the endosperm are marked by differential DNA methylation and are therefore epigenetically distinct. This epigenetic asymmetry is established in the gametes and maintained after fertilization by unknown mechanisms. In this manuscript, we have addressed the key question whether parentally inherited differential DNA methylation affects de novo targeting of chromatin modifiers in the early endosperm. Our data reveal that polycomb-mediated H3 lysine 27 trimethylation (H3K27me3) is preferentially localized to regions that are targeted by the DNA glycosylase DEMETER (DME), mechanistically linking DNA hypomethylation to imprinted gene expression. Our data furthermore suggest an absence of de novo DNA methylation in the early endosperm, providing an explanation how DME-mediated hypomethylation of the maternal genome is maintained after fertilization. Lastly, we show that paternal-specific H3K27me3-marked regions are located at pericentromeric regions, suggesting that H3K27me3 and DNA methylation are not necessarily exclusive marks at pericentromeric regions in the endosperm.
Keyphrases
- dna methylation
- genome wide
- gene expression
- copy number
- circulating tumor
- electronic health record
- transcription factor
- cancer therapy
- single molecule
- big data
- dna damage
- machine learning
- birth weight
- drug delivery
- nucleic acid
- dna repair
- oxidative stress
- physical activity
- amino acid
- long noncoding rna
- preterm birth
- weight loss
- gestational age
- circulating tumor cells