SCORe: SARS-CoV-2 Omicron Variant RBD-Binding DNA Aptamer for Multiplexed Rapid Detection and Pseudovirus Neutralization.
Lucy F YangNataly KacherovskyJoey LiangStephen J SalipanteSuzie H PunPublished in: Analytical chemistry (2022)
During the COVID-19 (coronavirus disease 2019) pandemic, several SARS-CoV-2 variants of concern emerged, including the Omicron variant, which has enhanced infectivity and immune invasion. Many antibodies and aptamers that bind the spike (S) of previous strains of SARS-CoV-2 either do not bind or bind with low affinity to Omicron S. In this study, we report a high-affinity S ARS- C oV-2 O micron R BD-binding aptam e r (SCORe) that binds Omicron BA.1 and BA.2 RBD with nanomolar K D1 . We employ aptamers SCORe.50 and SNAP4.74 in a multiplexed lateral flow assay (LFA) to distinguish between Omicron and wild-type S at concentrations as low as 100 pM. Finally, we show that SCORe.50 and its dimerized form SCOReD can neutralize Omicron S-pseudotyped virus infection of ACE2-overexpressing cells by >70%. SCORe therefore has potential applications in COVID-19 rapid diagnostics as well as in viral neutralization.
Keyphrases
- sars cov
- coronavirus disease
- respiratory syndrome coronavirus
- loop mediated isothermal amplification
- wild type
- escherichia coli
- single cell
- high throughput
- oxidative stress
- nucleic acid
- gold nanoparticles
- particulate matter
- gene expression
- single molecule
- binding protein
- copy number
- dna methylation
- sensitive detection
- cell proliferation
- genome wide
- pi k akt