A Non-invasive Liquid Biopsy Screening of Urine-Derived Exosomes for miRNAs as Biomarkers in Endometrial Cancer Patients.
Akhil SrivastavaKatherine MoxleyRachel RuskinDanny Natarajan DhanasekaranYan Daniel ZhaoRajagopal RameshPublished in: The AAPS journal (2018)
Exosomes have great potential to serve as a source of diagnostic and prognostic biomarkers for endometrial cancer (EC). Urine-derived exosomes from patients with EC and patients with symptoms of EC, but without established EC, were used to evaluate a unique miRNA expression profile. Of the 84 miRNA studied, 57 were amplified in qPCR, suggesting the differential packaging of miRNA in exosomes. Further, hsa-miR-200c-3p was identified to be enriched the most. Various bioinformatics and in silico tools were used to evaluate the biological significance of hsa-miR-200c-3p in EC. We conclude that differential miRNA in exosomes can be utilized for discovery of biomarker signatures and EC diagnosis; hsa-miR-200c-3p is one such candidate. Urine-derived exosomes pave the way for the development of non-invasive biomarkers.
Keyphrases
- endometrial cancer
- mesenchymal stem cells
- stem cells
- cell proliferation
- long non coding rna
- long noncoding rna
- end stage renal disease
- ejection fraction
- newly diagnosed
- chronic kidney disease
- small molecule
- prognostic factors
- peritoneal dialysis
- gene expression
- molecular docking
- ionic liquid
- genome wide
- risk assessment
- patient reported outcomes