Modulation of rifampicin-induced hepatotoxicity using poly(lactic-co-glycolic acid) nanoparticles: a study on rat and cell culture models.
Helal F HettaEsraa A AhmedAhmed G HemdanHeba Em El-DeekSaida Abd-ElregalNoura H Abd EllahPublished in: Nanomedicine (London, England) (2020)
Aim: Hepatotoxicity is the most serious adverse effect of rifampicin (RIF). We aimed to investigate the potential hepatoprotective effect of mannose-functionalized poly(lactic-co-glycolic acid)(PLGA)/RIF nanoparticles (NPs) in rats as a possible promising approach to minimize RIF-induced hepatotoxicity. Materials & methods: Mannose-functionalized PLGA/RIF NPs were fabricated and characterized in vitro, then the hepatoprotective effect of optimized NPs was studied on rat and cell culture models. Results: Following intraperitoneal administration of RIF NPs into rats, highly significant differences in levels of serum transaminases and oxidative stress markers, associated with significant differences in expression of Bax and Bcl-2 genes between NP- and free RIF-treated groups, revealing the hepatoprotective potential of NPs. Conclusion: RIF NPs may represent a promising therapeutic approach for tuberculosis via reducing dose frequency and consequently, RIF-induced hepatotoxicity.
Keyphrases
- pulmonary tuberculosis
- mycobacterium tuberculosis
- drug induced
- oxidative stress
- diabetic rats
- high glucose
- drug delivery
- emergency department
- gene expression
- induced apoptosis
- signaling pathway
- ischemia reperfusion injury
- genome wide
- dna methylation
- hepatitis c virus
- climate change
- human immunodeficiency virus
- drug release
- high resolution
- risk assessment
- heat stress
- mass spectrometry