Development and Characterization of Cellulose/Iron Acetate Nanofibers for Bone Tissue Engineering Applications.
Hamouda M MousaKamal Hany HusseinMostafa M SayedMohamed K Abd El-RahmanHeung-Myong WooPublished in: Polymers (2021)
In tissue engineering, design of biomaterial with a micro/nano structure is an essential step to mimic extracellular matrix (ECM) and to enhance biomineralization as well as cell biocompatibility. Composite polymeric nanofiber with iron particles/ions has an important role in biomineralization and collagen synthesis for bone tissue engineering. Herein, we report development of polymeric cellulose acetate (CA) nanofibers (17 wt.%) and traces of iron acetates salt (0.5 wt.%) within a polymeric solution to form electrospinning nanofibers mats with iron nanoparticles for bone tissue engineering applications. The resulting mats were characterized using field emission scanning electron microscopy (FESEM), transmission electron microscope (TEM), Fourier transform infrared (FTIR), thermal gravimetric analysis (TGA), differential scanning calorimetry (DSC), X-ray diffraction (XRD), and X-ray photoelectron spectroscopy (XPS). The resulted morphology indicated that the average diameter of CA decreased after addition of iron from (395 ± 30) to (266 ± 19) nm and had dense fiber distributions that match those of native ECM. Moreover, addition of iron acetate to CA solution resulted in mats that are thermally stable. The initial decomposition temperature was 300 °C of CA/Fe mat > 270 °C of pure CA. Furthermore, a superior apatite formation resulted in a biomineralization test after 3 days of immersion in stimulated environmental condition. In vitro cell culture experiments demonstrated that the CA/Fe mat was biocompatible to human fetal-osteoblast cells (hFOB) with the ability to support the cell attachment and proliferation. These findings suggest that doping traces of iron acetate has a promising role in composite mats designed for bone tissue engineering as simple and economically nanoscale materials. Furthermore, these biomaterials can be used in a potential future application such as drug delivery, cancer treatment, and antibacterial materials.
Keyphrases
- tissue engineering
- electron microscopy
- drug delivery
- extracellular matrix
- iron deficiency
- high resolution
- bone mineral density
- drug release
- cancer therapy
- protein kinase
- ionic liquid
- cell therapy
- bone regeneration
- single cell
- bone loss
- endothelial cells
- mesenchymal stem cells
- signaling pathway
- magnetic resonance imaging
- magnetic resonance
- induced apoptosis
- body composition
- solid state
- computed tomography
- stem cells
- mass spectrometry
- cell death
- single molecule
- postmenopausal women
- current status
- optical coherence tomography