DNAscent v2: detecting replication forks in nanopore sequencing data with deep learning.
Michael A BoemoPublished in: BMC genomics (2021)
This paper shows that DNAscent v2 is the new state-of-the-art in the high-throughput, single-molecule detection of replication fork dynamics. These improvements in DNAscent v2 mark an important step towards measuring DNA replication dynamics in large genomes with single-molecule resolution. Looking forward, the increase in accuracy in single-nucleotide resolution BrdU calls will also allow DNAscent v2 to branch out into other areas of genome stability research, particularly the detection of DNA repair.
Keyphrases
- single molecule
- dna repair
- high throughput
- deep learning
- atomic force microscopy
- living cells
- dna damage
- loop mediated isothermal amplification
- single cell
- real time pcr
- label free
- dna damage response
- big data
- artificial intelligence
- oxidative stress
- high resolution
- data analysis
- sensitive detection
- quantum dots
- high speed
- emergency medical