Characterization and application of porous gold nanoparticles as 2-photon luminescence imaging agents: 20-fold brighter than gold nanorods.
Joo H ParkJisoo ParkSuho KimSe-Hwa KimTae G LeeJae Y LeeJung-Sub WiPublished in: Journal of biophotonics (2017)
Two-photon nonlinear microscopy with the aid of plasmonic contrast agents is an attractive bioimaging technique capable of generating high-resolution images in 3 dimensions and facilitating targeted imaging with deep tissue penetration. In this work, physically synthesized gold nanoparticles containing multiple nanopores are used as 2-photon contrast agents and are reported to emit a 20-fold brighter 2-photon luminescence as compared to typical contrast agents, that is, gold nanorods. A successful application of our porous gold nanoparticles is experimentally demonstrated by in vitro nonlinear optical imaging of adipocytes at subcellular level.
Keyphrases
- high resolution
- gold nanoparticles
- reduced graphene oxide
- living cells
- single molecule
- magnetic resonance
- quantum dots
- mass spectrometry
- high speed
- fluorescent probe
- magnetic resonance imaging
- metabolic syndrome
- computed tomography
- skeletal muscle
- convolutional neural network
- high throughput
- cancer therapy
- silver nanoparticles
- tissue engineering
- fluorescence imaging