Molecular Bases of DNA Packaging in Bacteria Revealed by All-Atom Molecular Dynamics Simulations: The Case of Histone-Like Proteins in Borrelia burgdorferi.
Cécilia HognonSimon GaraudeJoanna TimminsChristophe J ChipotFrançois DehezAntonio MonariPublished in: The journal of physical chemistry letters (2019)
DNA compaction is essential to ensure the packaging of the genetic material in living cells and also plays a key role in the epigenetic regulation of gene expression. In both humans and bacteria, DNA packaging is achieved by specific well-conserved proteins. Here, by means of all-atom molecular dynamics simulations, including the determination of relevant free-energy profiles, we rationalize the molecular bases for this remarkable process in bacteria, illustrating the crucial role played by positively charged amino acids of a small histone-like protein. We also present compelling evidence that this histone-like protein alone can induce strong bending of a DNA duplex around its core domain, a process that requires overcoming a major free-energy barrier.
Keyphrases
- molecular dynamics simulations
- single molecule
- living cells
- dna methylation
- circulating tumor
- gene expression
- molecular docking
- cell free
- fluorescent probe
- molecular dynamics
- amino acid
- genome wide
- transcription factor
- nucleic acid
- circulating tumor cells
- solid phase extraction
- high resolution
- copy number
- electron transfer
- mass spectrometry
- tandem mass spectrometry