Carbonic Anhydrase Enzyme-MOFs Composite with a Superior Catalytic Performance to Promote CO2 Absorption into Tertiary Amine Solution.

Carbonic anhydrase (CA) enzyme-based absorption technology for CO2 capture has been intensively investigated. The main issue related to this novel technology is the activity and stability of the CA enzyme under the typical flue gas conditions. To address this issue, CA enzymes were embedded into zeolitic imidazolate framework (ZIF-L) nanoparticles to synthesize a novel CA/ZIF-L-1 composite. The composite exhibited a superior apparent catalytic activity (1.5 times higher) for CO2 absorption compared with their free counterparts, which was due to the synergistic enhancement of CO2 adsorption by support ZIF-L and enzymatic catalysis. The analyses of Fourier transform infrared spectroscopy and circular dichroism revealed that the CA enzyme's secondary structure was not significantly varied during the CA/ZIF-L-1 preparation, resulting in a high enzyme activity retention. Moreover, the CA/ZIF-L-1 possessed a high thermal stability and reusability due to the structural rigidity and confinement of ZIF-L scaffolds. Compared with the free enzyme, its thermal stability was improved by approximately 100% at 40 °C. After six cycles of reuse, CA/ZIF-L-1 still retained a relative activity of 134%. Therefore, the CA/ZIF-L-1 can be a good candidate to promote the CO2 capture in industrial application.