A whole blood intracellular cytokine assay optimised for field site studies demonstrates polyfunctionality of CD4+ T cells in acute scrub typhus.

Manutsanun InthawongNattapon PinthongAreerat ThaiprakhongTri WangrangsimakulPiyanate SunyakumthornJennifer HillPiengchan SonthayanonDaniel H ParisSusanna J DunachieBarbara Kronsteiner

Published in: PLoS neglected tropical diseases (2023)

In conclusion, this flow cytometry-based WBA is well-suited for use at field study sites, and enables the assessment of polyfunctional T cell responses to infectious agents and vaccines through delineation of antigen-specific cytokine secretion at the single cell level.

Keyphrases

flow cytometry
single cell
high throughput
liver failure
rna seq
respiratory failure
reactive oxygen species
drug induced
intensive care unit
aortic dissection
hepatitis b virus
clinical evaluation