Quantitative imaging of Candida utilis and its organelles by soft X-ray Nano-CT.
J LiuF LiL ChenYong GuanL TianY XiongG LiuY TianPublished in: Journal of microscopy (2017)
Soft X-ray microscopy has excellent characteristics for imaging cells and subcellular structures. In this paper, the yeast strain, Candida utilis, was imaged by soft X-ray microscopy and three-dimensional volumes were reconstructed with the SART-TV method. We performed segmentation on the reconstruction in three dimensions and identified several types of subcellular architecture within the specimen cells based on their linear absorption coefficient (LAC) values. Organelles can be identified by the correlation between the soft X-ray LAC values and the subcellular architectures. Quantitative analyses of the volume ratio of organelles to whole cell in different phases were also carried out according to the three-dimensional datasets. With such excellent features, soft X-ray imaging has a great influence in the field of biological cellular and subcellular research.
Keyphrases
- high resolution
- dual energy
- induced apoptosis
- mass spectrometry
- high speed
- cell cycle arrest
- computed tomography
- cell proliferation
- deep learning
- magnetic resonance imaging
- cell death
- biofilm formation
- staphylococcus aureus
- mesenchymal stem cells
- cell therapy
- image quality
- high throughput
- signaling pathway
- fluorescence imaging
- optical coherence tomography