Development of a new monoclonal antibody specific to mouse Vγ6 chain.
Shinya HatanoXin TunNaoto NoguchiDan YueHisakata YamadaXun SunMitsuru MatsumotoYasunobu YoshikaiPublished in: Life science alliance (2019)
There are seven Vγ gene segments in the TCR γ chain loci of mice. We developed monoclonal antibodies (mAbs) specific to the Vγ6 chain (Heilig & Tonegawa nomenclature). By immunizing Vγ4/6 KO mice with complementarity-determining region peptides in Vγ6 chains, we generated three hybridomas. These hybridomas produced mAbs capable of cell surface staining of Vγ6/Vδ1 gene-transfected T-cell line lacking TCR as well as of Vγ1- Vγ4- Vγ5- Vγ7- γδ T cells and the CD3high TCRδint γδ T cells in various organs. The location of Vγ6+ γδ T cells, which peaked in the newborn thymus, was associated with mTEC. In vivo administration of clone 1C10-1F7 mAb impaired protection against Klebsiella pneumoniae infection but ameliorated psoriasis-like dermatitis induced by imiquimod treatment. These new mAbs are useful to elucidate the development, location, and functions of Vγ6 γδ T cells in mice.
Keyphrases
- monoclonal antibody
- klebsiella pneumoniae
- high fat diet induced
- regulatory t cells
- genome wide
- cell surface
- escherichia coli
- multidrug resistant
- copy number
- wild type
- type diabetes
- gene expression
- dendritic cells
- atopic dermatitis
- dna methylation
- immune response
- metabolic syndrome
- transcription factor
- combination therapy
- smoking cessation
- replacement therapy