Zika virus replication and cytopathic effects in liver cells.
Kenneth E ShermanSusan D RousterLing X KongMatthew T AliotaJason T BlackardGary E DeanPublished in: PloS one (2019)
Zika virus (ZIKV) has emerged globally as an important pathogen, since it has been recognized as a cause of microcephaly and other neurologic processes and sequalae in newborns. The virus shares homology with Hepaciviruses and therefore may be a cause of hepatitis. We sought to characterize ZIKV replication in hepatocyte-derived cell lines. Huh7.5 and HepG2 cells were infected with ZIKV and replication potential was evaluated by multiple methods including plaque assay, qRT-PCR, negative-strand ZIKV RNA production, and ZIKV NS1 protein production. Growth curves in cells and supernatant were compared to replicative capacity in Vero cells. Overall, viral replication in both hepatocyte lines approximated that observed in the Vero cells. Cell cytopathology was observed after 3 days of infection and apoptosis markers increased. Transmission electron microscopy revealed evidence of viral capsids in cells and negative staining revealed ZIKV particles in the supernatant. Conclusions: Hepatocyte-derived cell lines are permissive for ZIKV replication and produce an overt cytopathic effect consistent with development of an acute viral hepatitis. Further evaluation of replication and injury is warranted.
Keyphrases
- zika virus
- dengue virus
- cell cycle arrest
- induced apoptosis
- aedes aegypti
- endoplasmic reticulum stress
- oxidative stress
- sars cov
- signaling pathway
- coronary artery disease
- risk assessment
- mesenchymal stem cells
- small molecule
- stem cells
- pi k akt
- cell therapy
- liver failure
- high throughput
- cell proliferation
- climate change
- hepatitis b virus
- amino acid
- protein protein