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Protein phosphatase 6 is a key factor regulating spermatogenesis.

Wen-Long LeiFeng HanMeng-Wen HuQiu-Xia LiangTie-Gang MengQian ZhouYing-Chun OuyangYi HouHeide SchattenZhen-Bo WangQian-Qian Sha
Published in: Cell death and differentiation (2019)
Protein phosphatase 6 (PP6) is a member of the PP2A-like subfamily, which plays a critical role in many fundamental cellular processes. We recently reported that PP6 is essential for female fertility. Here, we report that PP6 is involved in meiotic recombination and that germ cell-specific deletion of PP6 by Stra8-Cre causes defective spermatogenesis. The PP6-deficient spermatocytes were arrested at the pachytene stage and defects in DSB repair and crossover formation were observed, indicating that PP6 facilitated meiotic double-stranded breaks (DSB) repair. Further investigations revealed that depletion of PP6 in the germ cells affected chromatin relaxation, which was dependent on MAPK pathway activity, consequently preventing programmed DSB repair factors from being recruited to proper positions on the chromatin. Taken together, our results demonstrate that PP6 has an important role in meiotic recombination and male fertility.
Keyphrases
  • dna damage
  • gene expression
  • transcription factor
  • dna repair
  • germ cell
  • clinical trial
  • signaling pathway
  • oxidative stress
  • protein protein
  • small molecule
  • single cell
  • cell proliferation
  • genome wide identification