The effects of a globin blocker on the resolution of 3'mRNA sequencing data in porcine blood.

Kyu-Sang LimQian DongPamela MollJana VitkovskaGregor WiktorinStephanie BannisterDalia DaujotyteChristopher K TuggleJoan K LunneyGraham S PlastowJack C M Dekkers

Published in: BMC genomics (2019)

The effect of the GB on reducing the proportion of globin reads in porcine blood QuantSeq was demonstrated in three data sets. In addition to increasing the efficiency of sequencing non-globin mRNA, the GB for QuantSeq has an advantage that it does not require an additional step prior to or during library creation. Therefore, the GB is a useful tool in the quantification of whole gene expression profiles in porcine blood.

Keyphrases

electronic health record
single cell
big data
binding protein
genome wide
machine learning
deep learning