Evaluation of the clinical performance of a magnetic force-assisted electrochemical immunoassay for the detection of SARS-CoV-2 antigens.
Sung Jin JoSang-Hyun ShinJungrok KimSeungok LeeJehoon LeePublished in: PloS one (2021)
Rapid antigen (Ag) tests for the detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) provide quick results, do not require specialized technical skills or infrastructure, and can be used as a point-of-care method to prevent the spread of coronavirus disease (COVID-19). The performance of a magnetic force-assisted electrochemical immunoassay-based test, namely the MARK-B COVID-19 Ag test (BBB, Sungnam, Republic of Korea), was evaluated using 170 nasopharyngeal swab specimens and compared to that of RT-PCR and commercial rapid Ag test (STANDARD Q COVID-19 Ag Test, SD Biosensor, Suwon-si, Republic of Korea). The overall sensitivity and specificity of the MARK-B test were 90.0% (95% CI 79.4%-96.2%) and 99.0% (95% CI 95.0%-99.9%), respectively, with a kappa coefficient of 0.908. The correlations between the electrical current values of MARK-B and the Ct values of RT-PCR were -0.898 (E gene, 95% CI -0.938 to -0.834) and -0.914 (RdRp gene, 95% CI -0.948 to -0.860), respectively. The limit of detection of the MARK-B was measured using the viral culture reference samples and found to be 1 x 102 pfu/mL. The magnetic force-assisted electrochemical immunoassay-based Ag test can be used to rapidly detect SARS-CoV-2 infections, and the corresponding fully automated portable device can provide easy readability and semi-quantitative results.
Keyphrases
- sars cov
- respiratory syndrome coronavirus
- label free
- coronavirus disease
- loop mediated isothermal amplification
- quantum dots
- molecularly imprinted
- sensitive detection
- gold nanoparticles
- real time pcr
- single molecule
- highly efficient
- computed tomography
- magnetic resonance
- healthcare
- palliative care
- magnetic resonance imaging
- high throughput
- transcription factor
- blood brain barrier
- deep learning
- positron emission tomography
- health information
- social media
- toll like receptor
- fine needle aspiration
- liquid chromatography
- room temperature