Deep-Red/Near-Infrared Turn-On Fluorescence Probes for Aldehyde Dehydrogenase 1A1 in Cancer Stem Cells.
Masahiro OeKoji MikiYoshifumi UedaYasuo MoriAoi OkamotoYohei FunakoshiHironobu MinamiKouichi OhePublished in: ACS sensors (2021)
Accumulating evidence supports that cancer stem cells (CSCs) are responsible for cancer proliferation, metastasis, and therapy resistance; therefore, an effective strategy to identify and isolate CSCs is required urgently. Because of their low invasiveness and high signal/noise ratio, "turn-on" fluorescence probes working in the deep-red/near-infrared (DR/NIR) region are one of the most attractive yet undeveloped tools for CSC detection. Herein, we report DR/NIR turn-on fluorescence probes, CS5-A and CS7-A, targeted to aldehyde dehydrogenase 1A1 as an intracellular CSC marker. In contrast to the conventional "always-on" green-fluorescent ALDEFLUOR, we succeeded in generating high-contrast (signal/noise ratio > 8.3) and wash-free in vitro CSC imaging with the DR probe C5S-A. This probe can facilitate CSC isolation with minimal contamination by autofluorescence from other tissues through fluorescence-activated cell sorting. Furthermore, the NIR absorbance/emission and turn-on properties of C7S-A allow simple and rapid CSC detection in vivo within 15 min.
Keyphrases
- living cells
- cancer stem cells
- fluorescent probe
- single molecule
- loop mediated isothermal amplification
- energy transfer
- editorial comment
- magnetic resonance
- fluorescence imaging
- photodynamic therapy
- air pollution
- signaling pathway
- papillary thyroid
- risk assessment
- squamous cell carcinoma
- cell therapy
- single cell
- cancer therapy
- drug release
- gene expression
- real time pcr
- mass spectrometry
- sensitive detection
- lymph node metastasis
- replacement therapy
- contrast enhanced