Qualitative and Quantitative Evaluation of Rosavin, Salidroside, and p-Tyrosol in Artic Root Products via TLC-Screening, HPLC-DAD, and NMR Spectroscopy.
Hanna NikolaichukMarek StudzińskiMarek StankevičIrena M ChomaPublished in: Molecules (Basel, Switzerland) (2022)
Artic root is a well-known plant adaptogen with multipotential pharmacological properties. Thin-layer chromatography (TLC)-screening followed by diode-array high-performance liquid chromatography and nuclear magnetic resonance spectroscopy proved to be a reliable and convenient method for the simultaneous determination of the quality of various herbal raw materials and supplements. This combination allowed for comparing and differentiating arctic root samples as well as defining their authenticity. The study provided information on the chemical and biological properties of the seven chosen samples as well as qualitative and quantitative evaluation of the quality markers: rosavin, salidroside, and p-tyrosol. The absence of rosavin, salidroside, and p-tyrosol in three samples was detected using TLC screening and confirmed by HPLC-DAD and NMR. The paper highlighted the importance of quality control and strict regulation for herbal medicine supplements and preparations.
Keyphrases
- simultaneous determination
- high performance liquid chromatography
- tandem mass spectrometry
- liquid chromatography tandem mass spectrometry
- high resolution
- ultra high performance liquid chromatography
- liquid chromatography
- solid phase extraction
- quality control
- mass spectrometry
- ms ms
- gas chromatography
- systematic review
- climate change
- quality improvement
- magnetic resonance
- computed tomography
- social media
- single cell
- plant growth