Virtual education improved general understanding but required enhanced focus on topics like vaccination, pest management, and community biosecurity preparation for comprehensive knowledge. Both in-person and virtual learning resulted in an increase in agricultural biosecurity knowledge, with in-person learning exhibiting a higher proportion of significant knowledge gains. These findings emphasize the importance of diverse educational methods in preparing future agricultural workers to ensure safety and health in agricultural settings. Results were used to enhance the curricula content being made available as part of the Gearing Up for Safety program for young and beginning workers.