Determination of Flavonoids in Selected Scleranthus Species and Their Anti-Collagenase and Antioxidant Potential.

A new 5,7-dihydroxy-3'-methoxy-4'-acetoxyflavone-8- C - β -d-arabinopyranoside-2″- O -(4‴-acetoxy)-glucoside ( 6 ) and three known flavone C -glycosides-5,7,3',4'-tetrahydroxyflavone-6- C -xyloside-8- C - β -d-glucoside (lucenin-1) ( 7 ), 5,7,3'-trihydroxyflavone-6- C -glucoside-8- C - β -d-glucoside (vicenin-2) ( 8 ), and 5,7,4'-trihydroxy-3'-methoxyflavone-6- C - β -d-glucopyranoside-8- C - α -arabinopyranoside (chrysoeriol-6- C - β -d-glucopyranoside-8- C - α -arabinopyranoside) ( 9 )-were isolated from aerial parts of Scleranthus perennis L. (Caryophyllaceae). Their structures were determined through the use of comprehensive spectroscopic and spectrometric methods, and a method for the quantification of the major constituents of S. perennis and S. annuus L. was developed. Furthermore, the anti-collagenase and antioxidant activities of all isolated compounds obtained from extracts and fractions from both Scleranthus species were evaluated. The highest percentage of collagenase inhibition (at 400 µg/mL) was distinguished for methanolic extracts (22.06%, 32.04%) and ethyl acetate fractions (16.59%, 14.40%) from S. annuus and S. perennis . Compounds 6 - 9 displayed moderate inhibitory activity, with IC 50 values ranging from 39.59-73.86 µM.