P-MIRU, a polarized-spectral imaging system, reveals reflection information on the biological surface.

The reflection light forms the core of our visual perception of the world. We can obtain vast information by examining reflection light from biological surfaces, including pigment composition and distribution, tissue structure, and surface microstructure. However, because of the limitations in our visual system, the complete information in reflection light, which we term "reflectome," cannot be fully exploited. For example, we may miss reflection light information outside our visible wavelengths. In addition, unlike insects, we have virtually no sensitivity to light polarization. We can detect non-chromatic information lurking in reflection light only with appropriate devices. Though previous studies have designed and developed systems for specialized uses supporting our visual systems, we still do not have a versatile, rapid, convenient, and affordable system for analyzing broad aspects of reflection from biological surfaces. To overcome this situation, we developed P-MIRU, a novel multi-spectral and polarization imaging system for reflecting light from biological surfaces. The hardware and software of P-MIRU are open-source and customizable and thus can be applied for virtually any research on biological surfaces. Furthermore, P-MIRU is a user-friendly system for biologists with no specialized programming or engineering knowledge. P-MIRU successfully visualized multi-spectral reflection in visible/non-visible wavelengths and simultaneously detected various surface phenotypes of spectral polarization. P-MIRU system extends our visual ability and unveils information on biological surfaces. (217/250 words).