Wildfires are burning more acres annually, contributing to air pollution across the United States. Air pollutants, such as particulate matter (PM 2.5 ), have health implications for humans and animals, and are known to alter behavior in several species, but effects of wildfire PM 2.5 on dairy calf behavior are unknown. The present study aimed to understand how dairy calf standing and lying behavior is affected by wildfire PM 2.5 . Holstein heifer calves (n = 13) were monitored for the first 90 d of life, concurrent with the 2022 wildfire season. Hourly PM 2.5 concentrations and meteorological conditions, which were used to calculate temperature-humidity index (THI), were recorded. Wildfire and wind trajectory mapping was used to determine the contribution of wildfires to spikes in PM 2.5 . Calf activity data were recorded every minute using accelerometers and analyzed as total hourly and daily standing and lying times, standing and lying bouts, and duration of bouts. Additionally, the responses of calves to the initial 24-h period of each of 2 separate exposures to wildfire smoke were assessed. Wildfire PM 2.5 exposure was associated with reduced daily standing time and bout duration, increased daily total lying time, and increased, albeit not significantly, daily standing bouts. Percent of time standing hourly was increased, whereas percent of time lying hourly was decreased by wildfire PM 2.5 . The initial 24 h of each smoke exposure was characterized by decreased standing and increased lying time, but there was a greater change in behavior during the first event compared with the second event. These results indicate that exposure to wildfire PM 2.5 induces a behavioral response, which may diminish with repeated exposures. Future research should aim to understand the health and welfare implications of the behavioral responses to wildfire PM 2.5 .