A Capillary Electrophoresis Method Based on Molecularly Imprinted Solid-Phase Extraction for Selective and Sensitive Detection of Histamine in Foods.
Yixuan FanRunze YuYongfeng ChenYufeng SunGeoffrey I N WaterhouseZhixiang XuPublished in: Molecules (Basel, Switzerland) (2022)
In this study, a sensitive capillary electrophoresis (CE) method based on molecularly imprinted solid-phase extraction (MISPE) was proposed to determine histamine in foods. A molecularly imprinted polymer (MIP) synthesized by bulk polymerization was used as the MISPE adsorbent for the selective extraction of histamine. Under the optimal conditions, the MISPE-CE method possessed good linearity for histamine detection in the concentration range of 0.1-100.0 μg/L. The limit of detection and limit of quantification of the method were calculated to be 0.087 μg/L and 0.29 μg/L, respectively. The histamine in spiked rice vinegar and liquor samples were detected by the developed method with recoveries of 92.63-111.00%. The histamine contents in fish, prawn, pork, chicken breast and soy sauce samples were determined using the developed method and a high-performance liquid chromatography method, with no significant difference found between the two methods.
Keyphrases
- solid phase extraction
- molecularly imprinted
- high performance liquid chromatography
- liquid chromatography tandem mass spectrometry
- tandem mass spectrometry
- simultaneous determination
- gas chromatography mass spectrometry
- liquid chromatography
- capillary electrophoresis
- mass spectrometry
- gas chromatography
- ultra high performance liquid chromatography
- sensitive detection
- quantum dots
- high resolution mass spectrometry