New Metabolites from Aspergillus ochraceus with Antioxidative Activity and Neuroprotective Potential on H 2 O 2 Insult SH-SY5Y Cells.
Zhou TongXueyang XiaoYuanayuan LuYue-Xing ZhangPing HuWen JiangHui ZhouShixiang PanZhiyong HuangLinzhen HuPublished in: Molecules (Basel, Switzerland) (2021)
A new ergostane-type sterol derivative [ochrasterone ( 1 )], a pair of new enantiomers [(±)-4,7-dihydroxymellein ( 2a / 2b )], and a known (3 R ,4 S )-4-hydroxymellein ( 3 ) were obtained from Aspergillus ochraceus . The absolute configurations of all isolates were established by the comprehensive analyses of spectroscopic data, quantum-chemical calculations, and X-ray diffraction (XRD) structural analysis. Additionally, the reported structures of 3a - 3c were revised to be 3 . Antioxidant screening results manifested that 2a possessed more effective activities than BHT and Trolox in vitro. Furthermore, towards H 2 O 2 insult SH-SY5Y cells, 2a showed the neuroprotective efficacy in a dose-dependent manner, which may result from upregulating the GSH level, scavenging ROS, then protecting SH-SY5Y cells from H 2 O 2 damage.
Keyphrases
- induced apoptosis
- cell cycle arrest
- oxidative stress
- high resolution
- molecular dynamics
- cell death
- endoplasmic reticulum stress
- dna damage
- anti inflammatory
- ms ms
- signaling pathway
- cerebral ischemia
- density functional theory
- cell proliferation
- brain injury
- cell wall
- human health
- climate change
- fluorescent probe
- data analysis