Improvement in colistin-induced reproductive damage, apoptosis, and autophagy in testes via reducing oxidative stress by chrysin.
Emrah Hicazi AksuFatih Mehmet KandemirSefa KüçüklerAmdia MahamaduPublished in: Journal of biochemical and molecular toxicology (2018)
This study aimed to investigate the effect of chrysin on colistin-induced reproductive toxicity. Twenty-eight adult male Sprague-Dawley rats were divided into four groups of seven rats each. Group I received physiological saline for 7 days. Group II received 50 mg/kg/day chrysin for 7 days. Group III received a total dose of 73 mg/kg colistin for 7 days. Group IV received 50 mg/kg/day chrysin by an oral gavage after the colistin treatment. Colistin causes an increase in oxidative stress (OS) in the testis. Chrysin treatment significantly decreased the OS in the chrysin + colistin group compared with the colistin group. The highest caspase-3 and LC3B expression levels were found in the colistin group and these levels were statistically lower in the chrysin + colistin group. Colistin treatment caused a decrease in sperm motility and an increase in sperm abnormality. Chrysin treatment mitigated these side effects significantly. In conclusion, chrysin treatment can be beneficial against colistin-induced reproductive toxicity.
Keyphrases
- escherichia coli
- acinetobacter baumannii
- pseudomonas aeruginosa
- klebsiella pneumoniae
- oxidative stress
- multidrug resistant
- drug resistant
- gram negative
- diabetic rats
- dna damage
- cell death
- biofilm formation
- combination therapy
- signaling pathway
- endoplasmic reticulum stress
- cell proliferation
- long non coding rna
- replacement therapy
- cell cycle arrest
- candida albicans
- heat shock protein
- heat shock