Single-Molecule Sequencing Enables Long Cell-Free DNA Detection and Direct Methylation Analysis for Cancer Patients.

L Y Lois ChoyWenlei PengPeiyong JiangSuk Hang ChengStephanie C Y YuHuimin ShangO Y Olivia TseJohn WongVincent Wai-Sun WongGrace L H WongW K Jacky LamStephen L ChanRossa W K ChiuK C Allen ChanY M Dennis Lo

Published in: Clinical chemistry (2022)

A previously unidentified long cfDNA population was revealed in cancer patients. The presence and direct methylation analysis of these molecules open new possibilities for cancer liquid biopsy.

Keyphrases

single molecule
single cell
dna methylation
genome wide
papillary thyroid
living cells
atomic force microscopy
squamous cell
ultrasound guided
ionic liquid
loop mediated isothermal amplification
childhood cancer
sensitive detection
data analysis