Simultaneous imaging of lysosomal and mitochondrial viscosity during mitophagy using molecular rotors with dual-color emission.

Two molecular rotors with a single excitation and dual-color emission wavelengths were engineered for simultaneous imaging of lysosomal and mitochondrial viscosity. These two molecular rotors were highly sensitive to viscosity without dependence on pH or polarity, facilitating simultaneous imaging of lysosomal and mitochondrial viscosity during mitophagy in living cells.