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Directed movement toward, translocation along, penetration into and exit from vascular networks by breast cancer cells in 3D.

Deborah J WesselsClaude PujolNikash PradhanDaniel F LuscheLuis GonzalezSydney E KellyElizabeth M MartinEdward R VossYang-Nim ParkMichael DaileySonia L SuggSneha PhadkeAmani BashirBenjamin A Soll
Published in: Cell adhesion & migration (2022)
We developed a computer-assisted platform using laser scanning confocal microscopy to 3D reconstruct in real-time interactions between metastatic breast cancer cells and human umbilical vein endothelial cells (HUVECs). We demonstrate that MB-231 cancer cells migrate toward HUVEC networks, facilitated by filopodia, migrate along the network surfaces, penetrate into and migrate within the HUVEC networks, exit and continue migrating along network surfaces. The system is highly amenable to 3D reconstruction and computational analyses, and assessments of the effects of potential anti-metastasis monoclonal antibodies and other drugs. We demonstrate that an anti-RHAMM antibody blocks filopodium formation and all of the behaviors that we found take place between MB-231 cells and HUVEC networks.
Keyphrases
  • breast cancer cells
  • endothelial cells
  • small cell lung cancer
  • induced apoptosis
  • biofilm formation
  • high resolution
  • oxidative stress
  • cell death
  • mass spectrometry
  • endoplasmic reticulum stress