Integrated Osteoinductive Factors─Exosome@MicroRNA-26a Hydrogel Enhances Bone Regeneration.
Haizhu KuangJing MaXinyu ChiQichen FuQianzhe ZhuWeiling CaoPeng ZhangXin XiePublished in: ACS applied materials & interfaces (2023)
MicroRNAs (miRNAs) are a new therapeutic tool that can target multiple genes by inducing translation repression and target mRNA degradation. Although miRNAs have gained significant attention in oncology and in work on genetic disorders and autoimmune diseases, their application in tissue regeneration remains hindered by several challenges, such as miRNA degradation. Here, we reported Exosome@MicroRNA-26a (Exo@miR-26a), an osteoinductive factor that can be substituted for routinely used growth factors, which was constructed using bone marrow stem cell (BMSC)-derived exosomes and microRNA-26a (miR-26a). Exo@miR-26a-integrated hydrogels significantly promoted bone regeneration when implanted into defect sites; as the exosome stimulated angiogenesis, miR-26a promoted osteogenesis while the hydrogel enabled a site-directed release. Moreover, BMSC-derived exosomes further facilitated healthy bone regeneration by repressing osteoclast differentiation-related genes rather than damaging osteoclasts. Taken together, our findings demonstrate the promising potential of Exo@miR-26a for bone regeneration and provide a new strategy for the application of miRNA therapy in tissue engineering.
Keyphrases
- bone regeneration
- cell proliferation
- long non coding rna
- stem cells
- tissue engineering
- long noncoding rna
- bone marrow
- mesenchymal stem cells
- drug delivery
- wound healing
- genome wide
- working memory
- gene expression
- endothelial cells
- hyaluronic acid
- cell therapy
- vascular endothelial growth factor
- copy number
- replacement therapy
- binding protein
- genome wide analysis