A fluorescent naphthalimide NADH mimic for continuous and reversible sensing of cellular redox state.

Hemant SharmaNian Kee TanNatalie TrinhJia Hao YeoElizabeth J New Frederick M Pfeffer

Published in: Chemical communications (Cambridge, England) (2020)

A fluorescent, naphthalimide-based, NADH mimic has been synthesised as a reversible, biocompatible, "on-off" probe for the detection of changes in intracellular redox environment (both oxidation and reduction). Interconversion was confirmed by means of electrochemistry and also 1H NMR, UV-vis and fluorescence spectroscopy. The reversibility was also successfully detected in A549 cells under simulated redox stress.

Keyphrases

living cells
fluorescent probe
quantum dots
single molecule
label free
electron transfer
high resolution
induced apoptosis
solid state
magnetic resonance
cell cycle arrest
energy transfer
reactive oxygen species
endoplasmic reticulum stress
cell death
nitric oxide
real time pcr
drug release
stress induced