Removing the bottlenecks of cell culture metabolomics: fast normalization procedure, correlation of metabolites to cell number, and impact of the cell harvesting method.

Caroline MuschetGabriele MöllerCornelia PrehnMartin Hrabě de AngelisJerzy AdamskiJanina Tokarz

Published in: Metabolomics : Official journal of the Metabolomic Society (2016)

We offer a fast, robust, and validated normalization method for cell culture metabolomics samples and demonstrate the eligibility of the normalization of metabolomics data to the cell number. We show a cell line and metabolite-specific impact of the harvesting method on metabolite concentrations.

Keyphrases

single cell
mass spectrometry
cell therapy
ms ms
stem cells
electronic health record
artificial intelligence
big data
energy transfer