Phillygenin Suppresses Glutamate Exocytosis in Rat Cerebrocortical Nerve Terminals (Synaptosomes) through the Inhibition of Ca v 2.2 Calcium Channels.
Ming-Yi LeeTzu-Yu LinYa-Ying ChangKuan-Ming ChiuSu Jane WangPublished in: Biomedicines (2024)
Glutamate is a major excitatory neurotransmitter that mediates neuronal damage in acute and chronic brain disorders. The effect and mechanism of phillygenin, a natural compound with neuroprotective potential, on glutamate release in isolated nerve terminals (synaptosomes) prepared from the rat cerebral cortex were examined. In this study, 4-aminopyridine (4-AP), a potassium channel blocker, was utilized to induce the release of glutamate, which was subsequently quantified via a fluorometric assay. Our findings revealed that phillygenin reduced 4-AP-induced glutamate release, and this inhibitory effect was reversed by removing extracellular Ca 2+ or inhibiting vesicular transport with bafilomycin A1. However, exposure to the glutamate transporter inhibitor dl-threo-beta-benzyl-oxyaspartate (dl-TOBA) did not influence the inhibitory effect. Moreover, phillygenin did not change the synaptosomal membrane potential but lowered the 4-AP-triggered increase in intrasynaptosomal Ca 2+ concentration ([Ca 2+ ] i ). Antagonizing Ca v 2.2 (N-type) calcium channels blocked the inhibition of glutamate release by phillygenin, whereas pretreatment with the mitochondrial Na + /Ca 2+ exchanger inhibitor, CGP37157 or the ryanodine receptor inhibitor, dantrolene, both of which block intracellular Ca 2+ release, had no effect. The effect of phillygenin on glutamate release triggered by 4-AP was completely abolished when MAPK/ERK inhibitors were applied. Furthermore, phillygenin attenuated the phosphorylation of ERK1/2 and its major presynaptic target, synapsin I, a protein associated with synaptic vesicles. These data collectively suggest that phillygenin mediates the inhibition of evoked glutamate release from synaptosomes primarily by reducing the influx of Ca 2+ through Ca v 2.2 calcium channels, thereby subsequently suppressing the MAPK/ERK/synapsin I signaling cascade.
Keyphrases
- signaling pathway
- protein kinase
- oxidative stress
- pi k akt
- transcription factor
- cell proliferation
- cerebral ischemia
- machine learning
- multiple sclerosis
- single cell
- diabetic rats
- small molecule
- risk assessment
- respiratory failure
- subarachnoid hemorrhage
- electronic health record
- climate change
- drug induced
- blood brain barrier
- high throughput
- deep learning
- liver failure
- artificial intelligence
- big data
- amino acid
- acute respiratory distress syndrome
- cerebral blood flow
- prefrontal cortex