Multiplex miRNA assay using lanthanide-tagged probes and the duplex-specific nuclease amplification strategy.

Shixi ZhangRui LiuZhi XingSichun ZhangXinrong Zhang

Published in: Chemical communications (Cambridge, England) (2018)

A multiplex ICP-MS-based miRNA assay with duplex-specific nuclease amplification using bifunctional oligonucleotide probes was proposed. A multiplex assay of miR-141, let-7d, and miR-21 could be achieved simultaneously.

Keyphrases

high throughput
nucleic acid
cell proliferation
long non coding rna
single molecule
real time pcr
small molecule
long noncoding rna
single cell
mass spectrometry
living cells
multiple sclerosis
fluorescence imaging
dna binding
ms ms
photodynamic therapy
transcription factor